| 生物炭对岩溶区黄龙病脐橙园土壤N2O排放的影响 |
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| 关键词:biochar N2O emissions soil in karst area citrus Huanglongbing navel orange orchard |
| 基金项目:国家自然科学基金项目(编号: U20A2092; 42002106);中央级公益性科研院所基本科研业务费专项(编号: KK2005);中国地质调查局地质调查项目(编号: DD20190816; DD20190606) |
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| Hydrochemical Characteristics and Genesis of Deep Brines in Wangchang Area, Qianjiang Depression, Jianghan Basin |
| Soil nitrogen transformation of the Huanglongbing citrus orchard in the karst area has a particularity. The effects of soil amendment biochar on soil N2O emissions from the Huanglongbing citrus orchards in the karst areas and the microbial regulation pathways are currently unclear. In this study, the effects of biochar addition on soil N2O emissions, soil nitrogen net nitrification rates / mineralization rates, nitrification, and denitrification functional gene expression and their relationships were analyzed through cultivation experiments. The results showed that the addition of biochar altered the net nitrification rates / mineralization rates of soil nitrogen, abundance of denitrification-related functional genes nosZ, and nitrification-related functional genes AOA-amoA and AOB-amoA. When 2% biochar was added, it was the peak of most indicators, which was significantly different from the control. The addition of biochar reduced the average emission rates and cumulative emissions of N2O from the soil of the navel orange orchard in the karst area, but the differences among treatments were not significant. The DCA ordination test results showed that the N2O emission rates of different biochar addition treatments were mainly affected by the negative effects of NH+ 4-N content, nirK abundance, and nosZ abundance and the positive effects of NO– 3-N content and AOA-amoA abundance. To improve the effects of biochar on reducing soil N2O in the future, attention should be paid to increasing soil NH+ 4-N levels and the abundance of denitrification-related functional genes, while suppressing soil NO– 3-N levels and the abundance of nitrification-related functional genes, AOA-amoA. The results of this study provide a reference and support for the study of soil N2O emission reduction and mainatinence of the Huanglong citrus orchards in karst areas. |
| YUE Peng-peng,FU Can,JIANG Xiao-yu,WANG Rong,HUANG Ya-dan.2022.Hydrochemical Characteristics and Genesis of Deep Brines in Wangchang Area, Qianjiang Depression, Jianghan Basin[J].Acta Geoscientica Sinica,43(4):502-508. |
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