| 祁连山冻土区水合物DK3和DK6钻孔中微生物脂肪酸特征及意义 |
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| 关键词:fatty acid microbial diversity gas hydrate Qilian Mountain permafrost |
| 基金项目:天然气水合物勘查与试采工程国家专项项目“陆域天然气水合物环境监测及效应研究”(编号: GZHL20110327);中国地质大学(北京)基本科研业务费专项资金项目“祁连山冻土区天然气水合物区沉积物中微生物多样性研究”(编号: 2011PY0195) |
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| Discrimination of Microbial Diversity by Fatty Acid in Drill Hole DK3 and DK6 in a Gas Hydrate Area, Qilian Mountain Permafrost |
| The fatty acid (FA) method was used to evaluate microbial community diversity in (gas hydrate-bearing) Drill Hole DK3 and (hydrate-absent) Drill Hole DK6 in Qilian Mountain permafrost. 26 kinds of FA obtained in this study were divided into five types: BSFA, MUFA, CFA, PUFA and SSFA, with SFA being the dominant component. Microbial community of the cores from the two drill holes contain gram positive bacteria, gram negative bacteria and fungi classified from specificity FA. Principal component analysis and cluster analyses of FA from the two cores were performed using the PAST (palaeontological statistics, version 1.21) program. No significant difference of microbial components was observed between DK3 and DK6, but there did exist obvious difference of microbial components between gas hydrate-bearing (or methane anomaly) layer and hydrate-absent (or methane anomaly-absent) layer. The authors found that C16:1, C18:1 and methane anomaly have very good corresponding relationship. These phenomena prove from another side that methane concentration and gas hydrate may affect microbial habitats in Drill Hole DK3 and DK6. This is the first study of FA composition characteristics in permafrost sediments, and the results enrich the distribution range of FA and also expand the distribution depth of fungi; nevertheless, more experiments are needed to prove the specific mechanism. |
| JIAO Lu,SU Xin,HUANG Xia,LIU Hao-dong,HE Hao,JIANG Hong-chen,WANG Yong-li.2014.Discrimination of Microbial Diversity by Fatty Acid in Drill Hole DK3 and DK6 in a Gas Hydrate Area, Qilian Mountain Permafrost[J].Acta Geoscientica Sinica,35(5):599-607. |
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