| 祁连山冻土区水合物DK3和DK6钻孔中微生物脂肪酸特征及意义 |
| 点此下载全文 |
| 引用本文：焦露,苏新,黄霞,刘浩东,何浩,蒋宏忱,王永莉.2014.祁连山冻土区水合物DK3和DK6钻孔中微生物脂肪酸特征及意义[J].地球学报,35(5):599-607. |
| DOI：10.3975/cagsb.2014.05.10 |
| 摘要点击次数: 3353 |
| 全文下载次数: 3408 |
|
| 基金项目:天然气水合物勘查与试采工程国家专项项目“陆域天然气水合物环境监测及效应研究”(编号: GZHL20110327);中国地质大学(北京)基本科研业务费专项资金项目“祁连山冻土区天然气水合物区沉积物中微生物多样性研究”(编号: 2011PY0195) |
|
| 中文摘要:利用脂肪酸法分析祁连山冻土带水合物区DK3(含水合物)与DK6(不含水合物)钻孔岩心中微生物多样性。本研究获得C12到C24二十六种脂肪酸(FA), 可以分成直链饱和脂肪酸(SSFA), 支链饱和脂肪酸(BSFA), 单键不饱和脂肪酸(MUFA), 环丙烷脂肪酸(CFA)和多键不饱和脂肪酸(PUFA)五大类型, 其中SSFA相对含量最高。由于特异性的脂肪酸指示特异性的微生物类群, 得出两根岩心中微生物类群主要由革兰氏阳性菌、革兰氏阴性菌和真菌组成。运用PAST(Palaeontological statistics, version 1.21)软件对已获得的脂肪酸进行主成分分析和聚类分析, 得出DK3与DK6岩心的微生物组成并无显著性差异, 但是DK3岩心中含水合物层位与不含水合物层位微生物组成有差异, DK6岩心中有水合物异常的层位与无异常的层位有差异。发现C16:1和C18:1这两类脂肪酸与甲烷异常有很好的对应关系, 从另一方面证明了水合物异常影响微生物组成分布。本次研究首次获得冻土沉积物中FA组成特征, 丰富了FA的分布范围, 研究结果扩大了真菌的分布深度, 具体机理有待更多的实验证明。 |
| 中文关键词:脂肪酸 微生物多样性 天然气水合物 祁连山冻土带 |
| |
| Discrimination of Microbial Diversity by Fatty Acid in Drill Hole DK3 and DK6 in a Gas Hydrate Area, Qilian Mountain Permafrost |
|
|
| Abstract:The fatty acid (FA) method was used to evaluate microbial community diversity in (gas hydrate-bearing) Drill Hole DK3 and (hydrate-absent) Drill Hole DK6 in Qilian Mountain permafrost. 26 kinds of FA obtained in this study were divided into five types: BSFA, MUFA, CFA, PUFA and SSFA, with SFA being the dominant component. Microbial community of the cores from the two drill holes contain gram positive bacteria, gram negative bacteria and fungi classified from specificity FA. Principal component analysis and cluster analyses of FA from the two cores were performed using the PAST (palaeontological statistics, version 1.21) program. No significant difference of microbial components was observed between DK3 and DK6, but there did exist obvious difference of microbial components between gas hydrate-bearing (or methane anomaly) layer and hydrate-absent (or methane anomaly-absent) layer. The authors found that C16:1, C18:1 and methane anomaly have very good corresponding relationship. These phenomena prove from another side that methane concentration and gas hydrate may affect microbial habitats in Drill Hole DK3 and DK6. This is the first study of FA composition characteristics in permafrost sediments, and the results enrich the distribution range of FA and also expand the distribution depth of fungi; nevertheless, more experiments are needed to prove the specific mechanism. |
| keywords:fatty acid microbial diversity gas hydrate Qilian Mountain permafrost |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
|
